Biochemical activities of living tissues frequently tend to change the pH. Yet life depends upon maintaining a pH that will not inactivate the enzymes and other proteins working in that tissue. Can living tissues maintain a certain pH under normal conditions?
NOTE: This product is included in my Biochemistry Complete Unit Plan Bundle which can be viewed here: Biochemistry Bundled Unit Plan
This is the question that students will investigate in this lab. I use this lab in my Biology I classes, but it can easily be used for a middle school science class, or a more advanced Biology class. Students will observe that living cells (liver and potato) can definitely adapt and adjust their environments when drops of acid or base are added. Living cells produce buffers to maintain a stable pH. This lab never fails to work properly and my students are always amazed at the ability of a living cell to maintain a stable pH.
Materials required for this lab are: pH meter, 50 mL Beaker, .1N HCl, 250 mL beaker, .1N NaOH, 400 mL Beaker, Liver and potato homogenates, Graph Paper, Graduated cylinder
Your download will include a lab that is ready to be copied and passed out to your students. The student copy is a 4 page handout. Students can write on these handouts to fill in their data and answer all questions. I have also included all answers for the teacher, as well as teacher preparation notes for setting up this lab.
I have included both a pdf version and a Word document for your use.
Search words: Acid, Base, pH, buffer, enzymes, cells.
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