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One of the best things about taking a biology class is growing bacteria. All students love it! In this lab students will observe the effect that different antiseptics and disinfects have upon the growth of bacteria.
I use this lab in my Biology I classes, but it can easily be used for a middle school science class, or a more advanced Biology class.
Purpose:
1. To learn to grow bacteria on agar in a Petri dish.
2. To learn aseptic technique when working with bacteria.
3. To test the effectiveness of disinfectants and antiseptics upon the growth of bacteria.
Note: This product is also included in my bundled unit lesson plan that can be viewed by clicking this link: Viruses and Bacteria: Complete Teaching Unit containing 14 products
Materials required for this lab are: Three Petri dishes of nutrient agar, Bacteria spreader, Sharpie markers, Distilled water, Filter paper disks, Masking tape, Forceps, 2 disinfectants, Metric Ruler, 2 antiseptics, Isopropyl alcohol and Soil sample.
Your download will include a lab that is ready to be copied and passed out to your students. The student copy is a 4 page handout. Students can write on these handouts to fill in their data and answer all questions. I have also included all answers for the teacher, as well as teacher preparation notes for setting up this lab.
I have included both a pdf version and a Word document for your use.
You might also be interested in these products:
Viruses and Bacteria Powerpoint with Teacher and Student Notes
Viruses and Bacteria: Complete Teaching Unit containing 14 products
Test: Bacteria and Viruses
Quiz: Viruses
Lab: Microorganisms in School (Growing Bacteria)
Lab: Soil Communities (Testing for Bacteria in the Soil)
Viruses: Homework and Study guide
Bacteria Quiz or Homework
Bacteria Homework / Study Guide
Viruses Crossword Puzzle
Bacteria and Prokaryotes Crossword Puzzle
Viruses Powerpoint Jeopardy Review Game
Bacteria Powerpoint Jeopardy Review Game
Amy Brown - Science Stuff
I use this lab in my Biology I classes, but it can easily be used for a middle school science class, or a more advanced Biology class.
Purpose:
1. To learn to grow bacteria on agar in a Petri dish.
2. To learn aseptic technique when working with bacteria.
3. To test the effectiveness of disinfectants and antiseptics upon the growth of bacteria.
Note: This product is also included in my bundled unit lesson plan that can be viewed by clicking this link: Viruses and Bacteria: Complete Teaching Unit containing 14 products
Materials required for this lab are: Three Petri dishes of nutrient agar, Bacteria spreader, Sharpie markers, Distilled water, Filter paper disks, Masking tape, Forceps, 2 disinfectants, Metric Ruler, 2 antiseptics, Isopropyl alcohol and Soil sample.
Your download will include a lab that is ready to be copied and passed out to your students. The student copy is a 4 page handout. Students can write on these handouts to fill in their data and answer all questions. I have also included all answers for the teacher, as well as teacher preparation notes for setting up this lab.
I have included both a pdf version and a Word document for your use.
You might also be interested in these products:
Viruses and Bacteria Powerpoint with Teacher and Student Notes
Viruses and Bacteria: Complete Teaching Unit containing 14 products
Test: Bacteria and Viruses
Quiz: Viruses
Lab: Microorganisms in School (Growing Bacteria)
Lab: Soil Communities (Testing for Bacteria in the Soil)
Viruses: Homework and Study guide
Bacteria Quiz or Homework
Bacteria Homework / Study Guide
Viruses Crossword Puzzle
Bacteria and Prokaryotes Crossword Puzzle
Viruses Powerpoint Jeopardy Review Game
Bacteria Powerpoint Jeopardy Review Game
Amy Brown - Science Stuff
K-12 Subject:
Teaching Duration:
2 hours
Type of Resource:
Answer Key:
Included
# of Pages/Slides:
6
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1005.41 KB | 6 Pages
FEEDBACK
lwiseman1306
good
October 21, 2012 
Science Rules (TpT Seller)
I love this! I saw the results and cannot wait for my students to experience this 'wow! factor' lab that applies the scientific method, authentic learning experiences, inquiry and perhaps an eye-opener as to which products to use or not. Highly recommend!
February 29, 2012
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QUESTIONS AND ANSWERS:
lwiseman1306
what type of filter paper do you use? coffee filter?
October 23, 2012
Science Stuff (TpT Seller)
I use qualitative filter paper. It is a filter paper used in chemistry for qualitative analysis. It is medium speed filter paper. See Flinn Scientific catalog. Item AP5896.
Coffee filters will probably work just fine.
Coffee filters will probably work just fine.
October 23, 2012
lwiseman1306
What do you consider a bacteria spreader?
October 21, 2012
Science Stuff (TpT Seller)
A bacteria spreader is a simple device that is shaped like the letter "L". I have some that are plastic that I purchased with my other lab materials, and I have some that I made myself with glass tubing. I heated the glass in a Bunsen burner flame, and then bent the glass to a right angle to make an "L". The student holds the spreader at the top of the "L" so that the lower leg of the L is resting lightly on the agar. By moving the spreader across the surface of the agar, the inoculation sample is spread evenly across the surface of the agar. You might using a cotton swab or inoculating loop also, but my students tend to dig into the agar with these. Hope this helps! Please let me know if you need further clarification.
October 21, 2012
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I have been teaching for 27 years. I have taught Biology I, Honors Biology I, Chemistry, Honors Chemistry, Biology II, and AP Biology.
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