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Release of Heat in an Enzymatic Reaction (Catalase)

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Enzymes are required inside living cells to speed up chemical reactions. In this lab you will be measuring the amount of heat released as hydrogen peroxide is broken down into water and oxygen by the catalase in living cells.

Catalase is an enzyme that is commonly found in plant and animal tissues. The function of the enzyme catalase is to break down the chemical hydrogen peroxide inside living cells. Within cells, hydrogen peroxide is thought to be formed continually as an end product of biochemical reactions such as cellular respiration. Because it is toxic, or poisonous, it would soon kill the cell if it were not removed or broken down immediately.

Materials: Liver homogenate, 25 x 200 test tube, 3% hydrogen peroxide solution, 600 mL beaker, Thermometer, Insulating material, Graph paper, Stopwatch

Your 8 page download will include: Introduction, Materials, Procedure, Follow Up Questions, Student Data Pages, Answers, and Teacher Preparation Notes. You will receive both a pdf version and a Word document so that you can make changes if you wish.

NOTE: This product is also available in a bundled product called Enzymes, Catalysts and the Chemical Reactions of the Cell

NOTE: This product is included in my Biochemistry Complete Unit Plan Bundle which can be viewed here: Biochemistry Bundled Unit Plan

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Amy Brown - Science Stuff
K-12 Subject:
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Teaching Duration:
90 Minutes 
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8
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Release of Heat in an Enzymatic Reaction (Catalase)
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