Preparation of Chromosome Squashes Lab
Suitable for Honors Biology and AP Biology students.
In this lab, students will grow onion roots, and use the cells from the root tip to make a chromosome squash slide. Cells in the root tip are rapidly dividing by mitosis in order to make the root grow in length.
• To learn the technique of making chromosome squashes.
• To observe chromosomes in recently living cells.
• To determine if the rate of cell division correlates with the growth of the plant.
Your download will include Title, Purpose, Introduction, Materials, Safety Precautions, Procedure, Analysis Questions, Student Data Pages, Answers to all Questions, and Teacher Preparation Notes.
Materials Needed Include:
Onion sets, Toothpicks, Plastic cups, Razor blades, 70 % isopropyl alcohol, Test tubes, Rubber stoppers, Microscope slides, Cover slips, Microscopes, Syracuse dishes, Hydrochloric acid, 95% Ethyl alcohol, Carnoy's solution, Stopwatch, Aceto - orcein stain, Forceps
NOTE: This lab requires solutions that are potentially dangerous and must be handled with care.
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