Biology Lab: The Effect of the Enzyme Amylase on Starch

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Make your biology class more appealing and exciting by incorporating a variety of fun and interesting labs into your weekly lesson plans. This inquiry-based lab allows students to discover basic information about the functioning of enzymes within cells. Enzymes are biological catalysts that speed up the chemical reactions within cells. Without the aid of enzymes, the chemical reactions of the body would proceed so slow that the reaction would be of no use to the cell.

Purpose: In this lab, the student will observe the effect of the enzyme amylase on its substrate, starch. The student will perform various experiments with the enzyme amylase and will compile a list of facts concerning enzymes.

Materials: 0.3% amylase solution, 0.2% starch solution, 1% glucose solution, 1% sucrose solution, 1% maltose solution, Benedict's solution, Iodine solution, Biuret reagent, Glucose Test Strips, Stopwatch, Medicine droppers, Depression slides, Test tube rack, 9 test tubes (15 x 150), 50 mL beaker, 100 mL grad cylinder, 10 mL graduate cylinder, Hot plate, 600 mL beaker.

Students are given the task of trying to determine what happens to starch in the presence of the enzyme amylase. There are essentially four mini-experiments in the lab that will lead the student to the appropriate conclusion.

NOTE: This product is also available in a bundled product called Enzymes, Catalysts and the Chemical Reactions of the Cell

This lab is designed for a typical high school biology class for students in grades 9 - 12. It is appropriate for both standard and honors classes as well as for first or second year biology students. I have used this lab in both my freshman biology I class as well as my AP biology class.

Your download will include a 4-page handout for your students that includes: Title, Purpose, Materials List, Safety Precautions, Procedure, and Data Analysis Questions. Also included is a 4-page handout for the instructor that contains teaching tips and suggestions, information for how to prepare the solutions, lab set-up tips, and answers to all questions.

You will receive both a pdf and a Word document of this lab.

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Amy Brown Science Stuff
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1 hour

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Biology Lab: The Effect of the Enzyme Amylase on Starch
Biology Lab: The Effect of the Enzyme Amylase on Starch
Biology Lab: The Effect of the Enzyme Amylase on Starch
Biology Lab: The Effect of the Enzyme Amylase on Starch