This biology pre- and post-course test serves as a comprehensive check for understanding at the beginning and end of the school year. I packed twenty-eight multiple choice questions into a full-page document, which is ideal for teachers conscientious of the number of pages copied off. Topics covered include: scientific method, critiquing a published study, characteristics of life including homeostasis, hierarchy of life, cell chemistry, cell structure, cellular respiration, cell transport, cell division, protein synthesis, genetics, pedigree analysis, theories of evolution, taxonomy, microbiology, photosynthesis, ecology, adaptation, enzymes as catalysts, animal symmetry, and animal behavior. This document is editable. I included a two-page digitalized color-coated answer key.