This lab is designed for grade 9 students taking a Living Environment course (Biology). It uses scientific method to discover the pigments contained in green leaves. Teachers can use spinach, kale, parsley or other easily available greens. Students will predict the pigments then use Chromatography to test their hypotheses. Lab includes analysis and conclusion questions followed by application, reflection and evaluation questions using higher order thinking stems from bloom's taxonomy.
Thanks for looking. Note: solvent is water with a few drops of alcohol and a drop of detergent.