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This product offered by Spyglass Biology contains a DNA fingerprinting lab activity. The lab begins with a short story leading up to the crime scene scenario (I am featured in the story as the heroic biology teacher that helps solve the crime. You may want to change Mrs. Clelland to your name). Students then follow directions to determine who committed the crime. The lab simulates the procedure for prepping and running DNA samples through an electrophoresis gel. The students will first cut each DNA sample using 3 different restriction enzymes. Second, the students determine the length of each fragment and record the data in a table. Third, the students will translate the lengths by drawing corresponding bars on an “electrophoresis gel.” Fourth, students will glue the DNA fragments on an “electrophoresis gel” according to length. Lastly, there are several questions to answer before identifying whodunnit?
**FYI - Realistically, the guilty person's DNA should be an identical match, base by base, to the DNA extracted from the soda bottle. However, to force my students to complete the lab before being able to identify whodunnit, I had to modify the DNA sequences so that only the restriction sites were the same. This may warrant a discussion with your students after they complete the lab.
This product contains the student lab packet as a Word document, a PDF of the lab packet, and the answer key with score rubric.