Enzymes Mini-Unit: Worksheets, Graphing Activities, and Paper Substrate Lab

Grade Levels
9th - 12th
Resource Type
Formats Included
  • Zip
47 pages
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This high school biology mini-unit includes four separate teaching resources that teach the following topics:

Enzymes, Substrates, Active Sites, Allosteric Sites, and Inhibitors

Enzymatic Pathways

Introduction to Glycolysis

Free Energy Diagrams

Enzyme Rate versus Temperature Graphs

Enzyme Rate Calculations

I like to teach this enzyme mini-unit right before I teach aerobic cellular respiration, because enzymes and pathways really help students understand Glycolysis and the rest of Aerobic Cellular Respiration, beyond memorizing the reactants and products.

As in many of my products, I feel it is important to include content as well as question pages. So often if I find or buy a worksheet online, the content required for the questions is not included and it can be difficult for students to answer questions meant for a particular text or other resource. This is why I include what I call “Tutorials”, reading content with useful diagrams that can supplement textbooks and help teachers and students take a topic a little further.

Many of my questions are intended to get students to think deeper. These are not recall questions and students do not find them easy! They are challenging and I designed them this way. With lower level classes, the teacher may need to give students clues or lead them through a few questions to get them started. Only advanced students can complete these questions with no help.

This unit includes:

1. A set of tutorials and question sheets for Enzymes, Enzymatic Pathways, and Glycolysis.

Anatomy of an Enzyme and Questions (3 pages)

Enzymatic Pathway and Questions (3 pages)

Introduction of Glycolysis and Questions (3 pages)

Answer Keys for all Question Pages (4 pages)

2. A tutorial and question sheets for Free Energy Diagrams (4 pages plus 2 pages answer key)

3. A Paper Lab Activity that only requires teacher-cut paper copies of a simple “substrate”, three pairs of scissors, and a stopwatch/phone with a timer. Students can graph their results and calculate their “enzyme” rates. There are also two separate extension activities for more advanced classes, one adds an inhibitor to their enzymatic reactions and one encourages them to bring their understanding of activation energy and free energy diagrams to their experiment. (15 pages)

4. Firefly Luciferase – A Four Part Application Group Activity: Using a real life example of an enzyme, students calculate enzyme rates, they graph enzymatic rates vs temperature, they analyze a free energy diagram, and they model their own enzyme using modeling clay! You can do one activity at a time as a class or set up different learning areas of your classroom. This activity is very versatile and could be completed many different ways! (13 pages)

A total of 47 pages of content, questions, and activities for you!

**What buyers are saying about this resource!**

"Wonderful materials! Thank you so much for including the reading/background information. The writing is accessible to my students and the questions that go along with them are well done. The paper lab looks like a lot of fun, too! Thank you!"

"I love all these activities!"

Save 20% by buying this bundle instead of buying each resource individually!

You might also be interested in my Biotechnology: PCR, Restriction Enzymes, and Gel Electrophoresis mini-unit.

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If you have any questions or concerns, please reach out to us on the question and answer section of my store and we will get back to you quickly!

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© Bethany Lau 

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Total Pages
47 pages
Answer Key
Teaching Duration
1 Week
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