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Lab: Determining the Amount of Vitamin C in Fruit Juices

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How much Vitamin C is in Fruit Juices?

For students in Grades 8-12.

In this experiment the student will use a lab procedure known as a titration to determine the amount of Vitamin C found in a 6 ounce serving of each of the following juices: orange juice, pineapple juice, and apple juice.

This is a very versatile lab that can be used in several different classes for many different topics.
--Use this lab when teaching nutrition and digestion to your biology students.
--Can be used in an anatomy and physiology class.
--I use this lab in my chemistry classes when introducing the idea of titration and stoichiometry.

Materials Needed: Spot plate, Beral pipets or medicine droppers, White paper for background, Ascorbic acid standard solution, Apple juice, Orange juice, Pineapple juice, Starch solution, Iodine solution, Plastic toothpick (stirrer)

The four-page student handout includes: Introduction, Purpose, Materials List, Safety Precautions, Procedure, Data Table, Calculations and Analysis Questions.

The three-page teacher guide includes: Tips and suggestions, Instructions for mixing solutions, Solutions to the calculations, and answers to all questions.

All of my labs can be viewed by clicking this link.

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Total Pages
Answer Key
Teaching Duration
55 minutes
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