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# Lab - Effects of Tonicity on Cells (Observe & measure the effects of osmosis)

Product Description
Purpose: To observe and measure the effects of various concentrations of salt on potato squares.

Materials:
Hypertonic Solution (for entire class)
(20g salt/100mL water)
Isotonic Solution (for entire class)
(3g Salt/100mL water)
Hypotonic Solution (for entire class)
(Distilled or Tap Water)

Digital Weighing Scale
Potato Cubes
3 Paper Cups

Procedure:
1. Label each cup with your group number and other identifying labels. Label cup 1 “hypertonic”, cup 2 “isotonic”, and cup 3 “hypertonic”. Pour 10mL of each solution into each corresponding cup.
2. Weigh a potato square, record the initial mass (g),in the Group Data Table. Soak it in the HYPERTONIC SOLUTION.
3. Weigh a 2nd potato square, record its mass (g) and soak it in the ISOTONIC SOLUTION.
4. Weigh a 3rd potato square, record its mass (g) and soak it in the HYPOTONIC SOLUTION.
5. Let the 3 setups sit for 24 hours. Then, gently dab dry the potato soaked in the hypertonic solution, record its mass. Repeat this preceding step for the potato cubes in cups 2 and 3.
6. Calculate the change by subtracting “mass after 24 hrs” from the “initial mass”. Record your answers.
7. Consult with the other teams to complete the “Class Data Table”
Question/s: What is the effect of different tonicities (hypertonic, isotonic, and hypotonic solutions) on potato cells?
Hypotheses:
a. If potato cells are soaked in a hypertonic solution, then the potato cells
b. If potato cells are soaked in a isotonic solution, then the potato cells
c. If potato cells are soaked in a hyp0tonic solution, then the potato cells

GROUP DATA TABLE: Mass (grams) of Potato Cube
HYPERTONIC SOLUTION ISOTONIC
SOLUTION HYPOTONIC
SOLUTION
Initial mass (g) (g) (g)
After 24 hours (g) (g) (g)
Change in Mass
Subtract Day 2 from Day 1 mass indicate negative numbers (g) (g) (g)

CLASS DATA TABLE: Change in Mass (grams) of Potato Cube After Soaking
HYPERTONIC SOLUTION ISOTONIC
SOLUTION HYPOTONIC
SOLUTION
Team 1 (g) (g) (g)
Team 2 (g) (g) (g)
Team 3 (g) (g) (g)
Team 4 (g) (g) (g)
Team 5 (g) (g) (g)
Team 6 (g) (g) (g)
Average change in mass

Data Analysis:
1. Describe what happened to the potato cube soaked in hypertonic solution.
Explain this result.
2. Describe what happened to the potato cube soaked in isotonic solution.
Explain this result.
3. Describe what happened to the potato cube soaked in hypotonic solution.
Explain this result.
Conclusion: (Write your conclusions by referring to your hypotheses.)
Total Pages
2 pages
Answer Key
N/A
Teaching Duration
2 days
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