Leaf Pigment Chromatography Lab: Separating the pigments in leaves using paper chromatography.
The purpose of this lab is to teach students the lab technique called paper chromatography. Essential to the process of photosynthesis is a set of plant pigments whose function is to absorb the light energy from the sun. Chloroplasts use this energy to build organic molecules. Students will separate the pigments found in leaves using the technique of paper chromatography.
What is the purpose of this lab?
1. Student will learn the procedure of chromatography to separate the components of a mixture.
2. Student will separate the pigments found in leaves.
3. Students will calculate Rf values and answer critical thinking questions.
What materials are needed for this lab?
• Mortar and Pestle
• Fresh Leaves
• Chromatography paper,
• Capillary tubing
• 25 x 200 test tube,
• #4 or 5 rubber stopper
• Petroleum Ether
• Test tube rack.
Your download pages will include Title, Purpose, Introduction, Safety Precautions, Procedure, Student Data Pages, Follow Up Questions, Answer to all Questions, and Teacher Preparation Notes.
You will receive both an editable Word document as well as a pdf form of the lab.
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