Paper Chromatography Leaf Pigment Lab

Grade Levels
9th - 12th
Resource Type
Formats Included
  • Zip
11 pages
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This is a biology lab that involves separating leaf pigments using paper chromatography. The purpose of this lab is to teach students the lab technique called paper chromatography. Essential to the process of photosynthesis is a set of plant pigments whose function is to absorb the light energy from the sun. Chloroplasts use this energy to build organic molecules. Students will separate the pigments found in leaves using the technique of paper chromatography.

What is the purpose of this lab??

  1. Student will learn the procedure of chromatography to separate the components of a mixture.
  2. Student will separate the pigments found in leaves.
  3. Students will calculate Rf values and answer critical thinking questions.

What materials are needed for this lab?

  • Mortar and Pestle
  • Acetone
  • Fresh Leaves
  • Chromatography paper,
  • Capillary tubing
  • 25 x 200 test tube,
  • #4 or 5 rubber stopper
  • Petroleum Ether
  • Ruler
  • Calculator
  • Sand
  • Test tube rack.

Your download pages will include Title, Purpose, Introduction, Safety Precautions, Procedure, Student Data Pages, Follow Up Questions, Answer to all Questions, and Teacher Preparation Notes.

You will receive both an editable Word document as well as a pdf form of the lab.

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Total Pages
11 pages
Answer Key
Teaching Duration
2 hours
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to see state-specific standards (only available in the US).
Use a model to illustrate how photosynthesis transforms light energy into stored chemical energy. Emphasis is on illustrating inputs and outputs of matter and the transfer and transformation of energy in photosynthesis by plants and other photosynthesizing organisms. Examples of models could include diagrams, chemical equations, and conceptual models. Assessment does not include specific biochemical steps.
Follow precisely a complex multistep procedure when carrying out experiments, taking measurements, or performing technical tasks; analyze the specific results based on explanations in the text.
Follow precisely a complex multistep procedure when carrying out experiments, taking measurements, or performing technical tasks, attending to special cases or exceptions defined in the text.


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